Fine needle aspiration cytology puncture procedure

1 Routine disinfection, including the puncture site and the operator's hands.

2 The use of anesthetics should be avoided as far as possible for puncture in general parts, and satisfactory cell specimens should be obtained as much as possible. 2ml.

3 The punctureer uses the thumb and index finger of the left hand to fix the mass and the surrounding skin, and holds the needle in the right hand. The needle insertion direction is generally perpendicular to the body surface or at a 45-degree angle. The needle is inserted through the skin and then into the mass or lymph node. During operation, the needle should be inserted along the long axis of the tumor. The depth of needle insertion is 1/3 to 2/3 of the imaginary radius of the tumor or lymph node. The medulla of the lymph node and the center of the tumor should be avoided as much as possible, because these parts are prone to hemorrhage and necrosis, which may lead to the failure of puncture and sampling.

4 Fix the needle and the empty needle with the left hand, and pull the syringe core back to the 78ml mark with the right hand to keep the syringe under negative pressure. In order to absorb the cells in different parts, it can be pulled out to the subcutaneous, and then penetrated from the other direction; When the suction material fills the nipple of the syringe, quickly pull out the needle and apply pressure to stop the bleeding for 5-10 minutes.

minute.

5 Remove the needle after the needle is drawn out, pull the core back to inflate, then connect the needle, then push the specimen in the needle onto a clean glass slide, lay the needle flat and gently and evenly smear in the same direction, do not go back and forth in the same part Stir to avoid damaging the cells, and the smear should not be too thick.

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